Modulation of Lysosomal Cl− Mediates Migration and Apoptosis through the TRPML1 as a Lysosomal Cl− Sensor

Lysosomes are responsible for protein degradation and clearance in cellular recycling centers. It has been known that the lysosomal chloride level is enriched involved intrinsic function. However, mechanism by which levels can be sensed of chloride-mediated function unknown. In this study, we verified reduced acutely induced calcium release through TRPML1 repositioning toward juxtanuclear region. Functionally, low chloride-induced attenuated migration. addition, spontaneous exposure to dysregulated biogenesis subsequently delayed migration promoted apoptosis. Two chloride-sensing GXXXP motifs were identified. Mutations motif did not affect levels, there no changes migratory ability. demonstrated depletion induces reformation pool cancer progression, will assist improving therapeutic strategies accumulation-associated diseases or cell